RPS10 antibody, 0.57 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 6204 |
| Protein Name / Synonyms | RPS10 |
| Concentration (lot specific) | 0.57 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
DBA9 antibody, MGC88819 antibody, RPS10 antibody, 40S ribosomal protein S10 antibody, ribosomal protein S10 antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
19 kDa
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 151 (P46783) of RPS10
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
RPS10 antibody detects RPS10 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RPS10 protein stained by RPS10 antibody (107-10293) diluted at 1:500.
Blue: Hoechst 33343 staining.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RPS10 protein stained by RPS10 antibody (107-10293) diluted at 1:500.
Blue: Hoechst 33343 staining.
Sample (50 ug of whole cell lysate)
A: Mouse brain
12% SDS PAGE
107-10293 diluted at 1:1000
A: Mouse brain
12% SDS PAGE
107-10293 diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using RPS10(107-10293) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate)
A: A431
12% SDS PAGE
107-10293 diluted at 1:1000
A: A431
12% SDS PAGE
107-10293 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.