Anti-RPS3A Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Gene Id	6189
Protein Name / Synonyms	RPS3A
Concentration (lot specific)	1 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

FTE1 antibody, MFTL antibody, MGC23240 antibody, RPS3A antibody, 40S ribosomal protein S3a antibody, v-fos transformation effector protein 1 antibody, fte-1 antibody, ribosomal protein S3A antibody

Specificity

Species Reactivity

Human, Rat, Mouse

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

30 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 14 and 264 of RPS3A (Uniprot ID# P61247)

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

RPS3A antibody detects RPS3A protein at cytosol on mouse brain stem by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain stem.
RPS3A antibody (107-10505) dilution: 1:200.

RPS3A antibody detects RPS3A protein by Western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg GL261 whole cell lysate/extract
C. 30 µg C8D30 whole cell lysate/extract
D. 30 µg NIH-3T3 whole cell lysate/extract
E. 30 µg BCL-1 whole cell lysate/extract
F. 30 µg Raw264.7 whole cell lysate/extract
G. 30 µg C2C12 whole cell lysate/extract
12 % SDS-PAGE
RPS3A antibody (107-10505) dilution: 1:1000

RPS3A antibody detects RPS3A protein by Western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat2 whole cell lysate/extract
12 % SDS-PAGE
RPS3A antibody (107-10505) dilution: 1:1000

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ribosomal protein S3a(107-10505) antibody at 1:200 dilution.

Sample(30 ug whole cell lysate)
A: 293T
B: A431
C: H1299
D: HeLa S3
E: Hep G2
F: MOLT4
G: Raji
12% SDS PAGE
107-10505 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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