Anti-RPS3A (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P61247
Gene Id	6189
Gene Symbols	RPS3A
Protein Name / Synonyms	RPS3A (C-term)
Clonality	Polyclonal
Preparation	This RPS3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 228-254 amino acids from the C-terminal region of human RPS3A.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S3AE family of ribosomal proteins. It is located in the cytoplasm. Disruption of the gene encoding rat ribosomal protein S3a, also named v-fos transformation effector protein, in v-fos-transformed rat cells results in reversion of the transformed phenotype. Transcript variants utilizing alternative transcription start sites have been described. This gene is co-transcribed with the U73A and U73B small nucleolar RNA genes, which are located in its fourth and third introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Specificity

Predicted Reactivity

Mouse, Rat, Bovine, Monkey, Xenopus

Other Names

RPS3A; FTE1; MFTL; 40S ribosomal protein S3a; v-fos transformation effector protein

NCBI Accession #

NP_000997.1

Other Accession #

P49242; P97351; Q4R4Z6; Q56JV9; Q801S3; NP_000997.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

29945

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50

	RPS3A Antibody (C-term) (Cat# 102-16523) western blot analysis in Hela,A2058 cell line lysates (15ug/lane).This demonstrates the RPS3A antibody detected the RPS3A protein (arrow).
	RPS3A Antibody (C-term) (Cat# 102-16523) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RPS3A Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
	RPS3A Antibody (C-term) (Cat# 102-16523) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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