Anti-RPS6KA1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Rat
Host Species	Rabbit
Accession Number	Q15418
Gene Id	6195
Gene Symbols	RPS6KA1
Protein Name / Synonyms	RPS6KA1, HU-1, MAPKAPK1A, RSK, RSK1, p90Rsk, ribosomal protein S6 kinase A1
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IP
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Dilutions

WB 1:500 - 1:2000
IP 1:50 - 1:100

Format

Calculated MW

72kDa/81kDa/82kDa/83kDa

Observed MW

90kDa

Immunogen

A phospho specific peptide corresponding to residues surrounding S363 of human P90RSK

Modification

Phospho T359

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using Phospho-RPS6KA1-S363 antibody (144-61512) at 1:2000 dilution or RPS6KA1 antibody (144-015718). Hela cells were treated by ATP(5 mM) at 30? for 1 hour. C6 cells were treated by PMA/TPA (200 nM) at 37? for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - Phospho-P90RSK-S363 pAb (144-61512)

Expiration:

12 months from the date of shipment when stored properly.

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