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Anti-RPS6KA5 Antibody

Catalog #: 144-61526

Phospho-RPS6KA5-S376 pAb

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
O75582
Gene Id
9252
Gene Symbols
RPS6KA5
Protein Name / Synonyms
RPS6KA5, MSK1, MSPK1, RLPK, ribosomal protein S6 kinase A5
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IHC
Research AreaInflammation
Shipping TypeBlue ice
Storage-20°C

Description

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:100

Format

Calculated MW
61kDa/81kDa/89kDa
Observed MW
90kDa

Immunogen

A synthetic phosphorylated peptide around S376 of human RPS6KA5 (NP_004746.2).

Modification

Phospho S376

Storage

-20°C

Images

Western blot analysis of extracts of NIH/3T3 cells, using Phospho-RPS6KA5-S376 antibody (144-61526) at 1:2000 dilution. NIH/3T3 cells were treated by Anisomycin (25ug/ml) for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - Phospho-RPS6KA5-S376 pAb (144-61526)

Expiration:
12 months from the date of shipment when stored properly.