Synonyms
HU-1 antibody, MAPKAPK1A antibody, RSK antibody, RSK1 antibody, RPS6KA1 antibody, MAPK-activated protein kinase 1a antibody, MAPKAP kinase 1a antibody, p90-RSK 1 antibody, ribosomal S6 kinase 1 antibody, ribosomal protein S6 kinase alpha 1 antibody, dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1) antibody, MAPKAPK-1a antibody, RSK-1 antibody, 90 kDa ribosomal protein S6 kinase 1 antibody, S6K-alpha 1 antibody, MAP kinase-activated protein kinase 1a antibody, ribosomal protein S6 kinase alpha-1 antibody, S6K-alpha-1 antibody, p90S6K antibody, p90RSK1 antibody, ribosomal protein S6 kinase, 90kDa, polypeptide 1 antibody
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
83 kDa
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 303 and 527 (Q15418) of RSK1
Applications
Applications Tested
ICC/IF,Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

RSK1 antibody detects RPS6KA1 protein by Western blot analysis.
A. 30 µg HepG2 whole cell lysate/extract
7.5 % SDS-PAGE
RSK1 antibody (107-10564) dilution: 1:1000

RSK1 antibody detects RSK1 p90 protein at Cytoplasm and Nucleus by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RSK1 p90 protein stained by RSK1 antibody (107-10564) diluted at 1:500.
Blue: Hoechst 33343 staining.
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.