Anti-RSKB (MSK2) (C-term R321) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	O75676
Gene Id	8986
Gene Symbols	RPS6KA4
Protein Name / Synonyms	RSKB (MSK2) (C-term R321)
Clonality	Polyclonal
Preparation	This RSKB (MSK2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 322-354 amino acids from the C-terminal region of human RSKB (MSK2).
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting
Research Area	Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK). The calcium/calmodulin-dependent kinase (CAMK) group consists of 75 kinases regulated by Ca2+/CaM and close relative family (CAMK, CAMKL, DAPK, MAPKAPK).

Specificity

Other Names

RPS6KA4; MSK2; Ribosomal protein S6 kinase alpha-4; 90 kDa ribosomal protein S6 kinase 4; Nuclear mitogen- and stress-activated protein kinase 2; Ribosomal protein kinase B

NCBI Accession #

NP_001006945.1;NP_003933.1

Other Accession #

O75585

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

85606

Recommended Dilutions

WB: 1:1000

The anti-MSK2 Pab (Cat# 102-17066) is used in Western blot to detect MSK2 in placenta tissue lysate. Indirect immunofluorescence analysis showed that MSK2 is predominantly localized in the nucleus of parental (10T1/2) and oncogene-transformed (Ciras-3) mouse fibroblasts. Cells were co-stained with DAPI to visualize nucleus compartment. Data is kindly provided by B. Drobic and Dr. J. Davie from University of Manitoba (Winnipeg, Canada).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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