Anti-RUNX1 (S276) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q01196
Gene Id	861
Gene Symbols	RUNX1
Protein Name / Synonyms	RUNX1 (S276)
Clonality	Polyclonal
Preparation	This RUNX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 227-255 amino acids from human RUNX1.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF, FC
Research Area	Angiogenesis
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. The RUNX1 protein represents the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis.

Specificity

Other Names

RUNX1; AML1; CBFA2; Runt-related transcription factor 1; Acute myeloid leukemia 1 protein; Core-binding factor subunit alpha-2; Oncogene AML-1; Polyomavirus enhancer-binding protein 2 alpha B subunit; SL3-3 enhancer factor 1 alpha B subunit; SL3/AKV core-binding factor alpha B subunit

NCBI Accession #

NP_001001890.1;NP_001116079.1;NP_001745.2

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

48737

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; IF: 1:10-50; FC: 1:10-50

	RUNX1 Antibody (S276) (Cat# 102-15153) western blot analysis in U-937 cell line lysates (35ug/lane).This demonstrates the RUNX1 antibody detected the RUNX1 protein (arrow).
	Formalin-fixed and paraffin-embedded human brain tissue reacted with RUNX1 Antibody (S276) (Cat# 102-15153), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Confocal immunofluorescent analysis of RUNX1 Antibody (S276)(Cat# 102-15153) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
	RUNX1 Antibody (S276) (Cat# 102-15153) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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