Anti-RUNX3 (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q13761
Gene Id	864
Gene Symbols	RUNX3
Protein Name / Synonyms	RUNX3 (Center)
Clonality	Polyclonal
Preparation	This RUNX3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 168-197 amino acids from the Central region of human RUNX3.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

This gene encodes a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer. Multiple transcript variants encoding different isoforms have been found for this gene.

Specificity

Other Names

RUNX3; AML2; CBFA3; PEBP2A3; Runt-related transcription factor 3; Acute myeloid leukemia 2 protein; Core-binding factor subunit alpha-3; Oncogene AML-2; Polyomavirus enhancer-binding protein 2 alpha C subunit; SL3-3 enhancer factor 1 alpha C subunit; SL3/AKV core-binding factor alpha C subunit

NCBI Accession #

NP_001026850.1;NP_004341.1

Other Accession #

NP_001026850.1; NP_004341.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

44356

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; IF: 1:10-50

	Western blot analysis of lysates from HL-60, Jurkat cell line (from left to right), using RUNX3 Antibody (Center) (Cat# 102-12594). 102-12594 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
	RUNX3 Antibody (Center) (102-12594)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RUNX3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
	Fluorescent confocal image of Hela cell stained with RUNX3 Antibody (Center)(Cat# 102-12594).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RUNX3 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). RUNX3 immunoreactivity is localized to Nucleus and Cytoplasm significantly.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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