Anti-RUVBL1 (TIP49) (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q9Y265
Gene Id	8607
Gene Symbols	RUVBL1
Protein Name / Synonyms	RUVBL1 (TIP49) (Center)
Clonality	Polyclonal
Preparation	This RUVBL1 (TIP49) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 247-280 amino acids from the Central region of human RUVBL1 (TIP49).
Concentration (lot specific)	0.35 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

RUVBL1 possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity. It is a component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL1 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1 -CTNNB1 complex. High levels of autoantibodies against RUVBL1 are detected in sera of patients with autoimmune diseases such as polymyositis/dermatomyosistis and autoimmune hepatitis.

Specificity

Predicted Reactivity

Mouse, Rat, Zebrafish

Other Names

RUVBL1; INO80H; NMP238; TIP49; TIP49A; RuvB-like 1; 49 kDa TATA box-binding protein-interacting protein; 54 kDa erythrocyte cytosolic protein; INO80 complex subunit H; Nuclear matrix protein 238; Pontin 52; TIP49a; TIP60-associated protein 54-alpha

NCBI Accession #

NP_003698.1

Other Accession #

P60123; P60122; Q8AWW7

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

50228

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; FC: 1:10-50

	Western blot analysis of RUVBL1 (arrow) using rabbit polyclonal RUVBL1 Antibody (Cat# 102-14194). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RUVBL1 gene.
	RUVBL1 (TIP49) Antibody (Center) (Cat# 102-14194)immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RUVBL1 (TIP49) Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
	RUVBL1 (TIP49) Antibody (Center) (Cat# 102-14194) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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