Anti-Ryanodine Receptor Antibody

Product Description

Specifications

Size	50 µg
Estimated Lead Time	1-2 weeks
Species	Human
Specificity	This product reacts with the C-terminus cytoplasmic domain of ryanodine receptor. Ryanodine receptors have been shown to play critical roles in the intracellular Ca2+ signaling occurring during cell activation in muscle cells and non-muscle cells. This product reacts with ryanodine receptor (MW approx. 500kDa) isolated from a variety of cell types (e.g. lymphocytes, macrophages, granulocytes, fibroblasts, epithelial, endothelial cells, skeletal muscle, cardiac muscle and brain tissues).
Host Species	Mouse
Protein Name / Synonyms	Ryanodine Receptor
Concentration (lot specific)	IgG concentration 0.5 mg/ml
Recommended Applications	Immunohistology - Frozen, ELISA (dilution: , 1/100), Flow Cytometry, Immunoblotting, Immunofluorescence
Shipping Type	Blue ice
Storage	-20°C

Description

Immunohistology:

* Use indirect immunofluorescence. Visualization by confocal microscopy is required, as detection by standard fluorescent microscopy will not be adequate to detect the RyR. Additionally, fluorescent, not enzymatic, detection is required. Due to the intensity of confocal lasers, use of an anti-fading agent, such as DABCO, is strongly recommended.

Storage/Stability

Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.

Format

Supplied as:

Purified IgG - liquid

Formulation:

Phosphate buffered saline

Format Type:

Purified

Preparation

Immunogen:

Short synthetic polypeptide corresponding to the C-terminal domain of the ryanodine receptor.

Preservative:

0.09% Sodium azide

Expiration:

12 months from the date of shipment when stored properly.

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