Anti-RYK (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P34925
Gene Id	6259
Gene Symbols	RYK
Protein Name / Synonyms	RYK (C-term)
Clonality	Polyclonal
Preparation	This RYK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 561-591 amino acids from the C-terminal region of human RYK.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

RYK is an atypical member of the family of growth factor receptor protein tyrosine kinases, differing from other members at a number of conserved residues in the activation and nucleotide binding domains. This gene product belongs to a subfamily whose members do not appear to be regulated by phosphorylation in the activation segment. It has been suggested that mediation of biological activity by recruitment of a signaling-competent auxiliary protein may occur through an as yet uncharacterized mechanism. A nine nucleotide insertion in some transcripts results in the SLG variant. It is not established whether this is a product of alternative splicing or a second gene, since evidence for a second gene or pseudogene on chromosome 17 exists.

Specificity

Predicted Reactivity

Mouse

Other Names

RYK; JTK5A; Tyrosine-protein kinase RYK

NCBI Accession #

NP_001005861.1;NP_002949.2

Other Accession #

Q01887

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

67507

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	Western blot analysis of anti-hRYK-W576 Pab (Cat# 102-17551) pre-incubated without(lane 1) and with(lane 2) blocking peptide in 293 cell line lysate. hRYK-W576(arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Antigen Source

HUMAN

Storage/Stability

2-8^°C (short-term); -20^°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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