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Anti-S100b Antibody
Catalog #: 130-00023
Rabbit Anti-Human S100b Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | P04271 |
| Gene Id | 6285 |
| Gene Symbols | S100B |
| Clonality | Polyclonal |
| Immunogen | The immunogen was a synthetic peptide (S-100b). This antibody was produced from a rabbit immunized with this peptide conjugated with KLH. The IgG fraction was purified from rabbit serum by Protein G affinity chromatography. |
| Immunogen Sequence | CDFQEFMAFVAMVTTACHEFFEHE |
| Recommended Applications | ELISA (recommended work dilution= 1: 64, 000), Western Blotting (recommended work dilution= 1:1, 000), (see image) Western blot analysis of recombinant human S100b using anti-S100b antibody (1 :1, 000) |
| Reconstitution | Product is supplied as a powder obtained from lyophilization of purified antibody in PBS without preservatives. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml. |
| Research Area | Neuroscience |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
S100 calcium binding protein B or S100B is a protein of the S-100 protein family. S100 proteins are localized in the cytoplasm and nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include atleast 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3.
Specificity
This antibody can specifically bind to human S-100b. The Cross reactivity with mouse and rat S-100b was not tested
Storage
Store at 4°C if intended for use within one month, otherwise, store at -20°C to -80°C. The lyophilized antibody is stable for at least 18 months after the date of receipt when stored at -20°C to -80°C. After reconstitution, it can also be aliquoted and stored frozen at -20°C to -80°C in a manual defrost freezer for at least 6 months without detectable loss of activity. Upon reconstitution, the antibody can also be stored for 30 days at 4°C. Please avoid freeze-thaw cycles, as this will lower the activity of the antibody.
References
- Lin, (2008). "The astrocyte odyssey". Prog. Neurobiol. 86 (11): 34267. Baudier, J. and Cole, RD. (1988). "Interactions between the microtubule-associated tau proteins and S100b regulate tau phosphorylation by the Ca2+/calmodulin-dependent protein kinase II". J. Biol. Chem. 263 (12): 587683.
Expiration:
12 months from the date of shipment when stored properly.