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Anti-S100b Antibody, Biotinylated
Catalog #: 130-00023B-50
Rabbit anti-S100b Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P04271 |
| Gene Id | 6285 |
| Gene Symbols | S100B |
| Clonality | Polyclonal |
| Immunogen | The immunogen was a synthetic peptide (S-100b). This antibody was produced from a rabbit immunized with this peptide conjugated with KLH. The IgG fraction was purified from rabbit serum by Protein G affinity chromatography. |
| Immunogen Sequence | CDFQEFMAFVAMVTTACHEFFEHE |
| Isotype | IgG |
| Recommended Applications | ELISA (recommended work dilution= 1: 64, 000), Western Blotting (recommended work dilution= 1:1, 000), (see image) Western blot analysis of recombinant human S100b using anti-S100b antibody (1 :1, 000) |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Research Area | Neuroscience |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
S100 calcium binding protein B or S100B is a protein of the S-100 protein family. S100 proteins are localized in the cytoplasm and nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include atleast 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
This antibody can specifically bind to human S-100b. The Cross reactivity with mouse and rat S-100b was not tested
References
- Lin, (2008). "The astrocyte odyssey". Prog. Neurobiol. 86 (11): 34267. Baudier, J. and Cole, RD. (1988). "Interactions between the microtubule-associated tau proteins and S100b regulate tau phosphorylation by the Ca2+/calmodulin-dependent protein kinase II". J. Biol. Chem. 263 (12): 587683.
Expiration:
12 months from the date of shipment when stored properly.