Anti-S6K (RPS6KB1) (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P23443
Gene Id	6198
Gene Symbols	RPS6KB1
Protein Name / Synonyms	S6K (RPS6KB1) (Center)
Clonality	Polyclonal
Preparation	This S6K (RPS6KB1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 277-306 amino acids from the Central region of human S6K (RPS6KB1).
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	mTOR Signaling
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding the gene for RPS6KB1 and overexpression of this kinase are seen in some breast cancer cell lines.

Specificity

Predicted Reactivity

Mouse, Rat, Bovine, Rabbit

Other Names

RPS6KB1; STK14A; Ribosomal protein S6 kinase beta-1; 70 kDa ribosomal protein S6 kinase 1; Ribosomal protein S6 kinase I; Serine/threonine-protein kinase 14A; p70 ribosomal S6 kinase alpha

NCBI Accession #

NP_001258989.1;NP_003152.1

Other Accession #

P67999; P67998; Q8BSK8; Q6TJY3

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

59140

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	Western blot analysis of anti-RPS6KB1 Antibody (Center) Pab (Cat# 102-15369) in Jurkat cell line lysates (35ug/lane). RPS6KB1 Antibody (Center))(arrow) was detected using the purified Pab.
	Western blot analysis of anti-RPS6KB1 Antibody (Center) Pab (Cat# 102-15369) pre-incubated with and without blocking peptide (BP#NULL) in 293 cell line lysate. RPS6KB1 Antibody (Center)(arrow) was detected using the purified Pab.
	Western blot analysis of anti-RPS6KB1 Antibody (Center) Pab (Cat# 102-15369) in 293 cell line lysates transiently transfected with the RPS6KB1 gene (2ug/lane). RPS6KB1(arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human breast carcinoma reacted with RPS6KB1 Antibody (Center)(Cat# 102-15369), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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