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Anti-SAE1 Antibody
Catalog #: 107-11082
SAE1 antibody, 0.72 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Gene Id | 10055 |
| Protein Name / Synonyms | SAE1 |
| Concentration (lot specific) | 0.72 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
AOS1 antibody, FLJ3091 antibody, HSPC140 antibody, SUA1 antibody, UBLE1A antibody, SAE1 antibody, activator of SUMO1 antibody, sentrin/SUMO-activating protein AOS1 antibody, ubiquitin-like protein SUMO-1 activating enzyme antibody, SUMO-activating enzyme subunit 1 antibody, SUMO-1 activating enzyme E1 N subunit antibody, SUMO-1 activating enzyme subunit 1 antibody, ubiquitin-like 1-activating enzyme E1A antibody, SUMO1 activating enzyme subunit 1 antibody
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
38 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 3 and 247 of SAE1 (Uniprot ID# Q9UBE0)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
107-11082 diluted at 1:1000
A: Mouse brain
10% SDS PAGE
107-11082 diluted at 1:1000
SAE1 antibody detects SAE1 protein by Western blot analysis.
A. 30 µg Rat2 whole cell lysate/extract
10 % SDS-PAGE
SAE1 antibody (107-11082) dilution: 1:1000
A. 30 µg Rat2 whole cell lysate/extract
10 % SDS-PAGE
SAE1 antibody (107-11082) dilution: 1:1000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SAE1(107-11082) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using SAE1(107-11082) antibody at 1:100 dilution.
Sample (30 ug of whole cell lysate)
A: A431
B: H1299
10% SDS PAGE
107-11082 diluted at 1:1000
A: A431
B: H1299
10% SDS PAGE
107-11082 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.