Anti-SAP130 (C-term) Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Gene Id	23450
Protein Name / Synonyms	SAP130, C-term
Concentration (lot specific)	1 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

KIAA0017 antibody, RSE1 antibody, SAP130 antibody, SF3b130 antibody, STAF130 antibody, SF3B3 antibody, "pre-mRNA splicing factor SF3b, 130 kDa subunit antibody", splicing factor 3B subunit 3 antibody, pre-mRNA-splicing factor SF3b 130 kDa subunit antibody, spliceosome-associated protein 130 antibody, SAP 130 antibody, "splicing factor 3b, subunit 3, 130kDa antibody"

Specificity

Species Reactivity

Human

Format

Product Type

Primary antibody

Formulation

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

136 kDa

Immunogen

Synthetic peptide corresponding to a region within amino acids 1154 and 1217 of SAP130 (Uniprot ID# Q15393)

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000[suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of methanol-fixed HeLa, using SAP130(107-10624) antibody at 1:500 dilution.

SAP130 antibody [C3], C-term detects SAP130 protein by western blot analysis.
A. 30 µg HeLa whole cell extract
5 % SDS-PAGE
SAP130 antibody [C3], C-term (107-10624) dilution: 1:10000

Immunohistochemical analysis of paraffin-embedded Saos-2 xenograft, using SF3B3 (107-10624) antibody at 1:100 dilution.

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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