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ISO 13485:2016 cGMP
Anti-SARS-CoV-2 N-Protein Antibody
Catalog #: 130-10851
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | SARS-CoV-2 |
| Host Species | Mouse |
| Accession Number | QHD43423 |
| Protein Name / Synonyms | SARS-CoV-2 N-Protein |
| Clonality | Monoclonal |
| Clone | 1H6-A12-B12 |
| Immunogen | Immunogen was recombinant SARS-CoV-2 Nucleocapsid protein with C-terminal His-tag, derived from the transfected human HEK293 cells. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the immunogen. The IgG fraction of tissue culture supernatant was purified by Protein G/A affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution = 1:320, 000) |
| Reconstitution | Product is supplied as a powder obtained from lyophilization of purified antibody in 1 x PBS. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml. |
| Research Area | Infectious Disease, COVID-19 |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways.
Image
Lane: M: protein marker
1: 230-01104: 0.25 µg
2: 230-30164: 0.25 µg
3: 230-20409: 8.0 µl
130-10851 (mAb)
10 % SDS-PAGE
Goat anti-mouse IgG-HRP: 1:5000 (0.8 mg/ml).
Storage
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.