Anti-SART1 (internal) Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Gene Id	9092
Protein Name / Synonyms	SART1, Internal
Concentration (lot specific)	0.76 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

Ara1 antibody, HOMS1 antibody, MGC2038 antibody, SART1259 antibody, SNRNP110 antibody, Snu66 antibody, SART1 antibody, U4/U6.U5 tri-snRNP-associated protein 1 antibody, squamous cell carcinoma antigen recognised by T cells antibody, hSnu66 antibody, SART1(259) protein antibody, IgE autoantigen antibody, SNU66 homolog antibody, SART-1 antibody, small nuclear ribonucleoprotein 110kDa (U4/U6.U5) antibody, U4/U6.U5 tri-snRNP-associated 110 kDa protein antibody, hSART-1 antibody, squamous cell carcinoma antigen recognized by T cells 1 antibody, SART1(800) protein antibody, squamous cell carcinoma antigen recognized by T cells antibody

Specificity

Species Reactivity

Human, Mouse

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

90 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 137 and 447 of SART1 (Uniprot ID# O43290)

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using SART1(107-10383) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled at 1:2000.

Sample (30 ug of whole cell lysate)
A: Jurkat
B: Raji
C: K562
D: NCI-H929
7.5% SDS PAGE
107-10383 diluted at 1:5000

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
7.5% SDS PAGE
107-10383 diluted at 1:5000

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using SART1(107-10383) antibody at 1:500 dilution.

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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