Synonyms
C1orf3 antibody, SCAMP3 antibody, propin 1 antibody, secretory carrier-associated membrane protein 3 antibody, secretory carrier membrane protein 3 antibody
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
38 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 165 of SCAMP3 (Uniprot ID# O14828)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of methanol-fixed HeLa, using SCAMP3(107-10327) antibody at 1:1000 dilution.

Immunohistochemical analysis of paraffin-embedded adrenal gland, using SCAMP3(107-10327) antibody at 1:100 dilution.

Sample(30 µg of whole cell lysate)
A:MOLT4
12% SDS PAGE
107-10327 diluted at 1:2000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.