Anti-SCAP (N-term) Antibody

Product Description

Specifications

Size	200 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Mouse
Accession Number	Q12770
Gene Id	22937
Gene Symbols	SCAP
Protein Name / Synonyms	Sterol regulatory element-binding protein cleavage-activating protein, SCAP, SREBP cleavage-activating protein, SCAP, KIAA0199
Clonality	Monoclonal
Clone	1992C245.37.16
Isotype	Kappa, IgG1
Recommended Applications	Western Blotting
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Escort protein required for cholesterol as well as lipid homeostasis. Regulates export of the SCAP/SREBF complex from the ER upon low cholesterol. Formation of a ternary complex with INSIG at high sterol concentrations leads to masking of an ER-export signal in SCAP and retention of the complex in the ER. Low sterol concentrations trigger release of INSIG, a conformational change in the SSC domain of SCAP, unmasking of the ER export signal, recruitment into COPII-coated vesicles, transport to the Golgi complex, proteolytic cleavage of SREBF in the Golgi, release of the transcription factor fragment of SREBF from the membrane, its import into the nucleus and up-regulation of LDLR, INSIG1 and the mevalonate pathway (By similarity).

Other Information

Antigen Type

Synthesized Peptide

Format

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.

Calculated Molecular Weight (Da)

139729

Antigen Source

Human

Images

WB (1:4000)

All lanes : Anti-SCAP Antibody (N-term) at 1:4000 dilution Lane 1: HT-29 whole cell lysate Lane 2: LNCaP whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 140 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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