Synonyms
PACE-1 antibody, PACE1 antibody, RP1-97P20.2 antibody, SCYL3 antibody, SCY1-like protein 3 antibody, ezrin-binding protein PACE-1 antibody, protein-associating with the carboxyl-terminal domain of ezrin antibody, ezrin-binding partner PACE-1 (PACE-1) antibody, SCY1-like 3 (S. cerevisiae) antibody
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
83 kDa
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 80 and 549 (Q8IZE3) of SCY1 like 3
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SCY1 like 3(107-10387) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded H1299 xenograft , using SCY1 like 3(107-10387) antibody at 1:500 dilution.

Sample (30 ug of whole cell lysate)
A: A431
7.5% SDS PAGE
107-10387 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.