Anti-SCYL1 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q96KG9
Gene Id	57410
Gene Symbols	SCYL1
Protein Name / Synonyms	SCYL1 (N-term)
Clonality	Polyclonal
Preparation	This SCYL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-185 amino acids from the N-terminal region of human SCYL1.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

SCYL1 forms multimers following transfection into COS-7 cells. SCYL1 forms a 300-kD trimer using crosslinking reagents. Biochemical analysis revealed no phosphorylation or autophosphorylation activity.The 707-amino acid SCYL1 variant, variant 2, localized to centrosomes during mitosis. During interphase, fluorescence-tagged variant 2 localized in the cytoplasm as well as centrosomes. However, at the beginning of mitosis, the fluorescence appeared as a pair of bright nuclear foci that followed centrosome localization throughout mitosis, while maintaining diffuse cytoplasmic labeling. Endogenous variant 2 in HeLa cells showed a similar staining pattern. Centrosomal localization was independent of microtubules.

Specificity

Predicted Reactivity

Bovine

Other Names

SCYL1; CVAK90; GKLP; NTKL; TAPK; TEIF; TRAP; N-terminal kinase-like protein; Coated vesicle-associated kinase of 90 kDa; SCY1-like protein 1; Telomerase regulation-associated protein; Telomerase transcriptional element-interacting factor; Teratoma-associated tyrosine kinase

NCBI Accession #

NP_001041683.1;NP_065731.3

Other Accession #

A6QLH6

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

89631

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50

	SCYL1 Antibody (N-term) (Cat# 102-17187) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the SCYL1 antibody detected the SCYL1 protein (arrow).
	SCYL1 Antibody (N-term) (Cat# 102-17187)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SCYL1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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