Anti-SDHB (C-term) Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Gene Id	6390
Protein Name / Synonyms	SDHB, C-term
Concentration (lot specific)	1 mg/ml
Recommended Applications	ICC/IF, Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

FLJ92337 antibody, IP antibody, PGL4 antibody, SDH antibody, SDH1 antibody, SDH2 antibody, SDHIP antibody, SDHB antibody, succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial antibody, iron-sulfur subunit of complex II antibody, succinate dehydrogenase complex, subunit B, iron sulfur (Ip) antibody

Specificity

Species Reactivity

Human, Mouse

Format

Product Type

Primary antibody

Formulation

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

32 kDa

Immunogen

Synthetic peptide corresponding to a region within amino acids 221 and 280 of SDHB (Uniprot ID# P21912)

Applications

Applications Tested

ICC/IF,Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

SDHB antibody [C2C3], C-term detects SDHB protein by Western blot analysis.
A. 30 µg THP-1 whole cell lysate/extract
B. 30 µg NCI-H929 whole cell lysate/extract
12 % SDS-PAGE
SDHB antibody [C2C3], C-term (107-10467) dilution: 1:1000

SDHB antibody [C2C3], C-term detects SDHB protein by Western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
12 % SDS-PAGE
SDHB antibody [C2C3], C-term (107-10467) dilution: 1:1000

Immunofluorescence analysis of methanol-fixed HeLa, using SDHB(107-10467) antibody at 1:200 dilution.

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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