Anti-SDHD (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	O14521
Gene Id	6392
Gene Symbols	SDHD
Protein Name / Synonyms	SDHD (N-term)
Clonality	Polyclonal
Preparation	This SDHD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-42 amino acids from the N-terminal region of human SDHD.
Concentration (lot specific)	0.360 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF, FC
Research Area	Cardiovascular Disease, Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The subunit D protein is one of two integral membrane proteins anchoring the complex to the matrix side of the membrane. Mutations in SDHD have been linked to hereditary paraganglioma.

Specificity

Other Names

SDHD; SDH4; Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial; CII-4; QPs3; Succinate dehydrogenase complex subunit D; Succinate-ubiquinone oxidoreductase cytochrome b small subunit; Succinate-ubiquinone reductase membrane anchor subunit

NCBI Accession #

NP_001263432.1;NP_001263433.1;NP_001263435.1;NP_002993.1

Other Accession #

NP_002993.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

17043

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; IF: 1:10-50; FC: 1:10-50

	SDHD Antibody (N-term) (Cat# 102-11415) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the SDHD antibody detected the SDHD protein (arrow).
	SDHD Antibody (N-term) (Cat# 102-11415)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SDHD Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
	Confocal immunofluorescent analysis of SDHD Antibody (N-term)(Cat# 102-11415) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
	SDHD Antibody (N-term) (Cat# 102-11415) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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