Anti-Selenophosphate synthetase 1 Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Gene Id	22929
Protein Name / Synonyms	Selenophosphate synthetase 1
Concentration (lot specific)	0.92 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

MGC4980 antibody, SELD antibody, SPS antibody, SPS1 antibody, SEPHS1 antibody, selenophosphate synthetase 1 delta E2 antibody, selenium donor protein 1 antibody, selenophosphate synthetase 1 +E9a antibody, selenophosphate synthetase 1 major antibody, selenophosphate synthetase 1 +E9 antibody, selenophosphate synthetase 1 delta E8 antibody, selenophosphate synthase 1 antibody, selenide, water dikinase 1 antibody, selenophosphate synthetase 1 antibody

Specificity

Species Reactivity

Human

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

43 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 204 of Selenophosphate synthetase 1 (Uniprot ID# P49903)

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Selenophosphate synthetase 1 antibody detects SEPHS1 protein at nucleus on Colon Carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded Colon Carcinoma.
Selenophosphate synthetase 1 antibody (107-10685) dilution: 1:500.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Selenophosphate synthetase 1(107-10685) antibody at 1:200 dilution.

Sample(30 µg of whole cell lysate)
A:A431
B:MOLT4
12% SDS PAGE
107-10685 diluted at 1:500

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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