Synonyms
AXAM2 antibody, DKFZp762A2316 antibody, KIAA1331 antibody, SMT3IP2 antibody, SENP2 antibody, sentrin-specific protease 2 antibody, SMT3-specific isopeptidase 2 antibody, SUMO1/sentrin/SMT3 specific protease 2 antibody, sentrin/SUMO-specific protease SENP2 antibody, SUMO1/sentrin/SMT3 specific peptidase 2 antibody
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
68 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 1 and 62 of SENP2 (Uniprot ID# Q9HC62)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of paraformaldehyde-fixed A431, using SENP2(107-10921) antibody at 1:500 dilution.

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using SENP2(107-10921) antibody at 1:500 dilution.

Sample(30 ug whole cell lysate)
A:293T
B:MOLT4
7.5% SDS PAGE
107-10921 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.