Synonyms
MGC29667 antibody, SEST3 antibody, SESN3 antibody, sestrin-3 antibody, sestrin 3 antibody
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
57 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 21 and 296 of SESN3 (Uniprot ID# P58005)
Applications
Applications Tested
ICC/IF,Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

SESN3 antibody detects SESN3 protein by Western blot analysis.
A. 30 µg Raw264.7 whole cell lysate/extract
B. 30 µg C2C12 whole cell lysate/extract
7.5 % SDS-PAGE
SESN3 antibody (107-11016) dilution: 1:500

SESN3 antibody detects SESN3 protein by Western blot analysis.
A. 30 µg Rat2 whole cell lysate/extract
7.5 % SDS-PAGE
SESN3 antibody (107-11016) dilution: 1:1000

Immunofluorescence analysis of paraformaldehyde-fixed A549, using SESN3(107-11016) antibody at 1:200 dilution.

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
107-11016 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.