Anti-SET (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q01105
Gene Id	6418
Gene Symbols	SET
Protein Name / Synonyms	SET (N-term)
Clonality	Polyclonal
Preparation	This SET antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 47-75 amino acids from the N-terminal region of human SET.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone binding. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher.

Specificity

Predicted Reactivity

Mouse, Rat, Drosophila

Other Names

SET; Protein SET; HLA-DR-associated protein II; Inhibitor of granzyme A-activated DNase; PHAPII; Phosphatase 2A inhibitor I2PP2A; Template-activating factor I

NCBI Accession #

NP_001116293.1;NP_001234929.1;NP_001234930.1;NP_003002.2

Other Accession #

Q63945; Q9EQU5; P53997; NP_001116293.1; NP_003002.2

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

33489

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50

	SET Antibody (N-term) (Cat# 102-12555) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the SET antibody detected the SET protein (arrow).
	SET Antibody (N-term) (102-12555)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SET Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products