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Anti-SFRP1 Antibody, Biotinylated
Catalog #: 130-00007B-50
Chicken anti-SFRP1 Antibody Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Chicken |
| Accession Number | Q15797 |
| Gene Id | 4086 |
| Gene Symbols | SMAD1 BSP1 MADH1 MADR1 |
| Immunogen | The immunogen was a synthetic peptide. This antibody was produced from a rabbit immunized with this peptide conjugated with KLH. The IgG fraction was purified from rabbit serum by Protein G affinity chromatography. |
| Isotype | IgG |
| Recommended Applications | ELISA (recommended work dilution= 1: 32, 000) |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Research Area | Angiogenesis, Inflammation |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Secreted frizzled-related protein 1 (SFRP1) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP1 and SFRP5 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP1 is expressed in several human tissues, with the highest levels in heart. SFRP1 is a 35 kDa prototypical member of the SFRP family. It acts as a biphasic modulator of Wnt signaling, counteracting Wnt-induced effects at high concentrations and promoting them at lower concentrations
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
This antibody can specifically bind to human SFRP1. Cross reactivity with mouse or rat SFRP1 was not tested.
References
- Zhong, X. et al. (2007). "Regulation of secreted Frizzled-related protein-1 by heparin". J. Biol. Chem.282 (28): 2052333.
- Gumz, ML. et al. (2007). "Secreted frizzled-related protein 1 loss contributes to tumor phenotype of clear cell renal cell carcinoma". Clin. Cancer Res. 13 (16): 47409.
Expiration:
12 months from the date of shipment when stored properly.