RayBiotech

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Anti-SFRP5 (Center) Antibody

Catalog #: 102-17052

Rabbit Anti-Human SFRP5 (Center) Antibody, 400 ul

Product Description

Specifications

Size400 µL
Estimated Lead Time1-2 weeks
SpeciesHuman
Host SpeciesRabbit
Accession Number
Q5T4F7
Gene Id
6425
Gene Symbols
SFRP5
Protein Name / Synonyms
SFRP5 (Center)
ClonalityPolyclonal
PreparationThis SFRP5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 219-248 amino acids from the Central region of human SFRP5.
Concentration (lot specific)
0.5 mg/ml
Recommended ApplicationsWestern Blotting, IHC-P, FC
Shipping TypeBlue ice
Storage-20°C

Description

Introduction

Secreted frizzled-related protein 5 (SFRP5) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP5 and SFRP1 may be involved in determining the polarity of photoreceptor cells in the retina.

Specificity

Predicted Reactivity
Bovine
Other Names
SFRP5; FRP1B; SARP3; Secreted frizzled-related protein 5; Frizzled-related protein 1b; Secreted apoptosis-related protein 3
NCBI Accession #
NP_003006.2
Other Accession #
Q9XSC1

Format

Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
35563
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; FC: 1:10-50
Western blot analysis of SFRP5 Antibody (Center) (Cat# 102-17052) in K562 cell line lysates (35ug/lane). SFRP5 (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human hepatocarcinoma with SFRP5 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Flow cytometric analysis of K562 cells using SFRP5 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.