Anti-SFRS2 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q01130
Gene Id	6427
Gene Symbols	SRSF2
Protein Name / Synonyms	SFRS2 (N-term)
Clonality	Polyclonal
Preparation	This SFRS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-39 amino acids from the N-terminal region of human SFRS2.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

SFRS2 is necessary for the splicing of pre-mRNA. The protein is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. And it interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. It binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3' and can bind to beta-globin mRNA and commit it to the splicing pathway.

Specificity

Predicted Reactivity

Mouse, Rat, Bovine, Chicken, Pig

Other Names

SRSF2; SFRS2; Serine/arginine-rich splicing factor 2; Protein PR264; Splicing component, 35 kDa; Splicing factor SC35; Splicing factor, arginine/serine-rich 2

NCBI Accession #

NP_001182356.1;NP_003007.2

Other Accession #

Q6PDU1; Q06A98; Q62093; P30352; Q3MHR5

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

25476

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	Western blot analysis of anti-SFRS2 Antibody (N-term) (Cat# 102-15146) in K562 cell line lysates (35ug/lane).SFRS2(arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SFRS2 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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