SH3BP1 antibody, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 23616 |
| Protein Name / Synonyms | SH3BP1 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
ARHGAP43 antibody, SH3BP1 antibody, SH3 domain-binding protein 1 antibody, SH3 domain binding protein 1 antibody, 3BP-1 antibody, SH3-domain binding protein 1 antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
76 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 639 and 701 of SH3BP1
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of methanol-fixed HeLa, using SH3BP1(107-10699) antibody at 1:200 dilution.
SH3BP1 antibody detects SH3BP1 protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
SH3BP1 antibody (107-10699) dilution: 1:500.
Sample: Paraffin-embedded U87 xenograft.
SH3BP1 antibody (107-10699) dilution: 1:500.
Sample (30 ug of whole cell lysate)
A: Hela
B: JurKat
7.5% SDS PAGE
107-10699 diluted at 1:1000
A: Hela
B: JurKat
7.5% SDS PAGE
107-10699 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.