Anti-SIRT3 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q9NTG7
Gene Id	23410
Gene Symbols	SIRT3
Protein Name / Synonyms	SIRT3 (C-term)
Clonality	Polyclonal
Preparation	This SIRT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-279 amino acids from the C-terminal region of human SIRT3.
Concentration (lot specific)	0.500 mg/ml
Recommended Applications	Western Blotting, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

SIRT3 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The SIRT3 is included in class I of the sirtuin family.

Specificity

Other Names

SIRT3; SIR2L3; NAD-dependent protein deacetylase sirtuin-3, mitochondrial; Regulatory protein SIR2 homolog 3; SIR2-like protein 3

NCBI Accession #

NP_001017524.1;NP_036371.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

43573

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	Western blot analysis of lysates from 293, HepG2 cell line (from left to right), using SIRT3 Antibody (C-term)(Cat# 102-16695). 102-16695 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
	SIRT3 Antibody (C-term) (Cat# 102-16695) western blot analysis in 293 cell lysate (35ug/lane). This demonstrates that the SIRT3 antibody detected SIRT3 protein (arrow).
	SIRT3 Antibody (C-term) (Cat# 102-16695) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the SIRT3 antibody detected the SIRT3 protein (arrow).
	SIRT3 Antibody (C-term) (Cat# 102-16695) western blot analysis in KG-1 cell line lysates (35ug/lane).This demonstrates the SIRT3 antibody detected the SIRT3 protein (arrow).
	Western blot analysis of SIRT3 antibody (Cat# 102-16695) in Y79 cell line lysates (35ug/lane). SIRT3 (arrow) was detected using the purified Pab.
	Sirt3 Antibody (G265) (Cat# 102-16695) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the Sirt3 antibody detected the Sirt3 protein (arrow).
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.