Anti-Slap Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Gene Id	6503
Protein Name / Synonyms	Slap
Concentration (lot specific)	0.87 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

SLA1 antibody, SLAP antibody, SLA antibody, SLAP-1 antibody, src-like-adapter protein 1 antibody, hSLAP antibody, src-like-adapter antibody, Src-like-adaptor antibody

Specificity

Species Reactivity

Human, Mouse

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

31 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 276 of Slap (Uniprot ID# Q13239)

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of methanol-fixed A431, using Slap(107-11136) antibody at 1:200 dilution.

Slap antibody detects SLA protein at cytosol on gastric carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded gastric carcinoma.
Slap antibody (107-11136) dilution: 1:500.

Slap antibody detects SLA protein at cytosol on mouse stomach by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
Slap antibody (107-11136) dilution: 1:500.

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
12% SDS PAGE
107-11136 diluted at 1:2000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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