Anti-SLC8A1 (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P32418
Gene Id	6546
Gene Symbols	SLC8A1
Protein Name / Synonyms	SLC8A1 (Center)
Clonality	Polyclonal
Preparation	This SLC8A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 296-325 amino acids from the Central region of human SLC8A1.
Concentration (lot specific)	0.460 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Research Area	Cardiovascular Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

In cardiac myocytes, Ca(2+) concentrations alternate between high levels during contraction and low levels during relaxation. The increase in Ca(2+) centration during contraction is primarily due to release of Ca(2+) from intracellular stores. However, some Ca(2+) also enters the cell through the sarcolemma (plasma membrane). During relaxation, Ca(2+) is sequestered within the intracellular stores. To prevent overloading of intracellular stores, the Ca(2+) that entered across the sarcolemma must be extruded from the cell. The Na(+)-Ca(2+) exchanger is the primary mechanism by which the Ca(2+) is extruded from the cell during relaxation. In the heart, the exchanger may play a key role in digitalis action. The exchanger is the dominant mechanism in returning the cardiac myocyte to its resting state following excitation.

Specificity

Predicted Reactivity

Rat, Bovine

Other Names

SLC8A1; CNC; NCX1; Sodium/calcium exchanger 1; Na(+)/Ca(2+)-exchange protein 1

NCBI Accession #

NP_001106271.1;NP_001106272.1;NP_001106273.1;NP_001239553.1;NP_066920.1

Other Accession #

Q01728; P70414; P48765

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

108547

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; FC: 1:10-50

	Western blot analysis of SLC8A1 Antibody (Center) (Cat# 102-18085) in HL-60(lane 1), K562(lane 2) cell line lysates (35ug/lane). SLC8A1 (arrow) was detected using the purified Pab.
	SLC8A1 Antibody (Center) (Cat# 102-18085) IHC analysis in formalin fixed and paraffin embedded mouse brain followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SLC8A1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
	SLC8A1 Antibody (Center) (Cat# 102-18085) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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