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Anti-SMAC Antibody, Biotinylated
Catalog #: RB-02-0029B-50
Rabbit anti-SMAC Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q9NR28 |
| Gene Id | 56616 |
| Gene Symbols | DIABLO SMAC |
| Clonality | Polyclonal |
| Immunogen | The immunogen was a synthetic peptide (SMAC). This antibody was produced from a rabbit immunized with this peptide conjugated with KLH. The IgG fraction was purified from rabbit serum by Protein G affinity chromatography. |
| Immunogen Sequence | CEERAESEEEAYLRED |
| Isotype | IgG |
| Recommended Applications | ELISA (recommended work dilution= 1: 64, 000), Western Blotting (recommended work dilution= 1: 1, 000) |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Second mitochondria-derived activator of caspases, SMAC is a mitochondrial protein that promotes cytochrome-c dependent activation by eliminating the inhibition via IAP - a protein that negatively regulates apoptosis or programmed cell death. Smac is normally a mitochondrial protein but is found in the cytosol when a cell is primed for apoptosis by the final execution step of caspase activation. Smac is the second protein in the apoptosis link, along with cytochrome c, that promotes apoptosis by activating caspases. SMAC is an inhibitor of apoptosis protein (IAP)-binding protein. This mitochondrial protein enters the cytosol when cells undergo apoptosis, and it moderates the caspase inhibition of IAPs.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
This antibody can specifically bind to human SMAC. The cross reactivity with mouse and rat SMAC was not tested.
References
- McNeish IA, et al. (2003). "Expression of Smac/DIABLO in ovarian carcinoma cells induces apoptosis via a caspase-9-mediated pathway". Exp Cell Res 286 (2): 18698.
- Yu J, et al. (2007). "SMAC/Diablo mediates the proapoptotic function of PUMA by regulating PUMA-induced mitochondrial events". Oncogene 26 (29): 418998.
Expiration:
12 months from the date of shipment when stored properly.