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Anti-Smad1 Antibody, Biotinylated
Catalog #: 130-00004B-50
Rabbit anti-Smad1 Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q15797 |
| Gene Id | 4086 |
| Gene Symbols | SMAD1 BSP1 MADH1 MADR1 |
| Clonality | Polyclonal |
| Immunogen | A phosphorylated synthetic peptide corresponding to the region of amino acids containing serine 214 of human SMAD1 protein was used as immunogen. This antibody was produced from a rabbit immunized with this peptide conjugated with KLH. The IgG fraction was purified from rabbit serum by Protein G affinity chromatography. |
| Isotype | IgG |
| Recommended Applications | ELISA (recommended work dilution= 1: 32, 000), Western Blotting (recommended work dilution= 1: 1, 000) |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Research Area | Angiogenesis, Inflammation, TGF-beta Signaling |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Mothers against decapentaplegic homolog 1 also known as SMAD family member 1 or SMAD1 is a protein in humans encoded by the SMAD1 gene. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
This antibody can specifically bind to human SMAD1 only when phosphorylated at Ser214. Cross reactivity with mouse or rat SMAD1 was not tested.
References
- Hoodless, PA. et al. (1996). "MADR1, a MAD-related protein that functions in BMP2 signaling pathways". Cell 85 (4): 489500.
- Riggins, GJ. et al. (1996). "Mad-related genes in the human". Nat. Genet. 13 (3): 3479.
Expiration:
12 months from the date of shipment when stored properly.