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Anti-SMAD2 Antibody
Catalog #: 102-17305
Rabbit Anti-SMAD2 Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | Q15796 |
| Gene Id | 4087 |
| Gene Symbols | SMAD2 |
| Protein Name / Synonyms | SMAD2 |
| Clonality | Polyclonal |
| Preparation | This SMAD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 97-125 amino acids from human SMAD2. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | IF, Western Blotting, FC |
| Research Area | Cardiovascular Disease, TGF-beta Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
The protein belongs to the SMAD, a family of proteins similar to the proteins of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin.
Specificity
Predicted Reactivity
Rat, Zebrafish, Bovine, Chicken, Drosophila, Pig
Other Names
SMAD2; MADH2; MADR2; Mothers against decapentaplegic homolog 2; JV18-1; Mad-related protein 2; SMAD family member 2
NCBI Accession #
NP_001003652.1;NP_005892.1
Other Accession #
O54835; Q9JIW5; O15198; Q9R1V3; P97454; Q99717; Q9W7E7; Q56I99; P84025; P84024; Q8BUN5; P84022; P84023; O70436; Q62432; Q9I9P9; Q1W668; P97588; P70340; Q15797; Q9I8V2; Q1JQA2; P42003
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
52306
Recommended Dilutions
IF: 1:100; WB: 1:1000; FC: 1:10-50
 | Fluorescent confocal image of SY5Y cells stained with SMAD2 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with 102-17305 SMAD2 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min). Note the highly specific localization of the SMAD2 mainly to the nucleus. |
 | Western blot analysis of SMAD2 Antibody (Cat# 102-17305) in NIH-3T3 cell line lysates (35ug/lane). SMAD2 (arrow) was detected using the purified Pab. |
 | SMAD2 Antibody (Cat# 102-17305) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.