Anti-SMAD4 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q13485
Gene Id	4089
Gene Symbols	SMAD4
Protein Name / Synonyms	SMAD4 (C-term)
Clonality	Polyclonal
Preparation	This SMAD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 400-428 amino acids from the C-terminal region of human SMAD4.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF, FC
Research Area	Cardiovascular Disease, Cancer, TGF-beta Signaling
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

SMAD4 is the common SMAD (co-SMAD)mediator of signal transduction by TGF-beta (transforming growth factor). It promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. It may act as a tumor suppressor.

Specificity

Predicted Reactivity

Mouse, Rat, Bovine, Pig

Other Names

SMAD4; DPC4; MADH4; Mothers against decapentaplegic homolog 4; Deletion target in pancreatic carcinoma 4; SMAD family member 4

NCBI Accession #

NP_005350.1

Other Accession #

O70437; Q9GKQ9; P97471; Q1HE26

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

60439

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; IF: 1:10-50; FC: 1:10-50

	Western blot analysis of SMAD4 (arrow) using rabbit polyclonal SMAD4 Antibody (C-term) (Cat# 102-17603). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SMAD4 gene (Lane 2) .
	Formalin-fixed and paraffin-embedded human skeletal muscle reacted with SMAD4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Fluorescent confocal image of Hela cell stained with SMAD4 Antibody (C-term)(Cat# 102-17603).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SMAD4 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). SMAD4 immunoreactivity is localized to Cytoplasm and Nucleus significantly.
	SMAD4 Antibody (C-term) (Cat# 102-17603) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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