Anti-SMAD4 (T277) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q13485
Gene Id	4089
Gene Symbols	SMAD4
Protein Name / Synonyms	SMAD4 (T277)
Clonality	Polyclonal
Preparation	This SMAD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 255-284 amino acids from human SMAD4.
Concentration (lot specific)	0.390 mg/ml
Recommended Applications	Western Blotting, IF
Research Area	Cardiovascular Disease, Cancer, TGF-beta Signaling
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

SMAD4 is the common SMAD (co-SMAD)mediator of signal transduction by TGF-beta (transforming growth factor). It promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. It may act as a tumor suppressor.

Specificity

Predicted Reactivity

Mouse, Rat

Other Names

SMAD4; DPC4; MADH4; Mothers against decapentaplegic homolog 4; Deletion target in pancreatic carcinoma 4; SMAD family member 4

NCBI Accession #

NP_005350.1

Other Accession #

O70437; P97471

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

60439

Recommended Dilutions

WB: 1:1000; IF: 1:10-50

	Western blot analysis of SMAD4 Antibody (T277)(arrow) using rabbit polyclonal SMAD4 Antibody (T277) (Cat# 102-17602). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SMAD4 gene (Lane 2) (Origene Technologies).
	Confocal immunofluorescent analysis of SMAD4 Antibody (T277)(Cat# 102-17602) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).