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Anti-SMAD9 (Center) Antibody
Catalog #: 102-12713
Rabbit Anti-Human SMAD9 (Center) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | O15198 |
| Gene Id | 4093 |
| Gene Symbols | SMAD9 |
| Protein Name / Synonyms | SMAD9 (Center) |
| Clonality | Polyclonal |
| Preparation | This SMAD9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 200-228 amino acids from the Central region of human SMAD9. |
| Concentration (lot specific) | 0.450 mg/ml |
| Recommended Applications | Western Blotting, IF |
| Research Area | TGF-beta Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
The protein encoded by this gene is a member of the SMAD family, which transduces signals from TGF-beta family members. The encoded protein is activated by bone morphogenetic proteins and interacts with SMAD4. Two transcript variants encoding different isoforms have been found for this gene.
Specificity
Other Names
SMAD9; MADH6; MADH9; Mothers against decapentaplegic homolog 9; Madh6; SMAD family member 9
NCBI Accession #
NP_001120689.1;NP_005896.1
Other Accession #
NP_005896.1; NP_001120689.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
52493
Recommended Dilutions
WB: 1:1000; IF: 1:10-50
 | SMAD9 Antibody (Center) (Cat# 102-12713) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the SMAD9 antibody detected the SMAD9 protein (arrow). |
 | Fluorescent confocal image of MCF-7 cell stained with SMAD9 Antibody (Center)(Cat# 102-12713). MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SMAD9 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).SMAD9 immunoreactivity is localized to cytoplasm and nucleus significantly. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.