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Anti-SMARCC2 Antibody
Catalog #: 144-01967
SMARCC2 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q8TAQ2 |
| Gene Id | 6601 |
| Gene Symbols | SMARCC2 |
| Protein Name / Synonyms | SMARCC2, BAF170, CRACC2, Rsc8, SWI/SNF complex subunit SMARCC2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and contains a predicted leucine zipper motif typical of many transcription factors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:200 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
124kDa/126kDa/132kDa
Observed MW
171kDa
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 150-250 of human SMARCC2 (NP_620706.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using SMARCC2 antibody (144-01967) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat kidney using SMARCC2 antibody (144-01967) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate cancer using SMARCC2 antibody (144-01967) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.