Synonyms
CDLS2 antibody, DKFZp686L19178 antibody, DXS423E antibody, KIAA0178 antibody, MGC138332 antibody, SB1.8 antibody, SMC1 antibody, SMC1L1 antibody, SMC1alpha antibody, SMCB antibody, SMC1A antibody, SMC protein 1A antibody, SMC1 structural maintenance of chromosomes 1-like 1 antibody, SMC-1A antibody, "SMC1 (structural maintenance of chromosomes 1, yeast)-like 1 antibody", SMC-1-alpha antibody, structural maintenance of chromosomes protein 1A antibody, segregation of mitotic chromosomes 1 antibody, structural maintenance of chromosomes 1A antibody
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
143 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 248 of SMC1A (Uniprot ID# Q14683)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using SMC1A(107-10862) antibody at 1:100 dilution.

Sample (30 ug of whole cell lysate)
A: Molt-4
7.5% SDS PAGE
107-10862 diluted at 1:5000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.