Anti-SMURF1 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q9HCE7
Gene Id	57154
Gene Symbols	SMURF1
Protein Name / Synonyms	SMURF1 (N-term)
Clonality	Polyclonal
Preparation	This SMURF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-96 amino acids from the N-terminal region of human SMURF1.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Members of the transforming growth factor-beta (TGFB) family signal through type I and type II serine/threonine-kinase receptors, which in turn activate the SMAD signaling pathway. Bone morphogenetic protein (BMP) receptors target SMAD1, SMAD5, and SMAD8, whereas receptors for activin and TGFB (e.g., ACVR1 and TGFBR1, respectively) target SMAD2 and SMAD3. Phosphorylation of these receptor-regulated SMADs induces their association with the common-partner SMAD, SMAD4. Smurf1, a HECT domain E3 ubiquitin ligase, regulates cell polarity and protrusive activity and is required to maintain the transformed morphology and motility of a tumor cell. Atypical protein kinase C-zeta (PKC2), an effector of the Cdc42/Rac1-PAR6 polarity complex, recruits Smurf1 to cellular protrusions, where it controlled the local level of RhoA. Smurf1 thus links the polarity complex to degradation of RhoA in lamellipodia and filopodia to prevent RhoA signaling during dynamic membrane movements.

Specificity

Predicted Reactivity

Zebrafish, Xenopus

Other Names

SMURF1; KIAA1625; E3 ubiquitin-protein ligase SMURF1; SMAD ubiquitination regulatory factor 1; SMAD-specific E3 ubiquitin-protein ligase 1

NCBI Accession #

NP_001186776.1;NP_065162.1;NP_851994.1

Other Accession #

Q2TAS2; A2A5Z6; Q9HAU4; A9JRZ0; Q9PUN2; Q9CUN6

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

86114

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50

	Western blot analysis of anti-SMURF1 Pab (Cat# 102-14965) in mouse kidney tissue lysate (35ug/lane). SMURF1(arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with hSMURF1-W81 (Cat# 102-14965), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Formalin-fixed and paraffin-embedded human Testis tissue reacted with hSMURF1-W81 (Cat# 102-14965), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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