RayBiotech

3607 Parkway Ln Ste 200 Norcross, Georgia 30092

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770-729-2992 | +1-888-494-8555

ISO 13485:2016 cGMP

Anti-SNRPB Antibody

Catalog #: 144-61328

SNRPB Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
P14678
Gene Id
6628
Gene Symbols
SNRPB
Protein Name / Synonyms
SNRPB, CCMS, COD, SNRPB1, Sm-B/B', SmB/B', SmB/SmB', snRNP-B, small nuclear ribonucleoprotein polypeptides B and B1
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting
Shipping TypeBlue ice
Storage-20°C

Description

Background

The protein encoded by this gene is one of several nuclear proteins that are found in common among U1, U2, U4/U6, and U5 small ribonucleoprotein particles (snRNPs). These snRNPs are involved in pre-mRNA splicing, and the encoded protein may also play a role in pre-mRNA splicing or snRNP structure. Autoantibodies from patients with systemic lupus erythematosus frequently recognize epitopes on the encoded protein. Two transcript variants encoding different isoforms (B and B') have been found for this gene.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW
23kDa/24kDa/30kDa
Observed MW
30kDa

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SNRPB (NP_003082.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using SNRPB antibody (144-61328) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 180s.
Western blot - SNRPB antibody (144-61328)

Expiration:
12 months from the date of shipment when stored properly.