Synonyms
D16S2531E antibody, EST25263 antibody, SNT2B2 antibody, SNT3 antibody, SNTL antibody, SNTB2 antibody, 59 kDa dystrophin-associated protein A1 basic component 2 antibody, beta-2-syntrophin antibody, syntrophin-3 antibody, "dystrophin-associated protein A1, 59kD, basic component 2 antibody", "syntrophin, beta 2 (dystrophin-associated protein A1, 59kDa, basic component 2) antibody"
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
58 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 174 and 540 of SNTB2 (Uniprot ID# Q13425)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of methanol-fixed HeLa, using SNTB2(107-10810) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft , using SNTB2(107-10810) antibody at 1:500 dilution.

Sample (30 ug of whole cell lysate)
A: Hep G2
7.5% SDS PAGE
107-10810 diluted at 1:3000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.