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Anti-SOD3 (N-term) Antibody
Catalog #: 102-16985
Rabbit Anti-Human SOD3 (N-term) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P08294 |
| Gene Id | 6649 |
| Gene Symbols | SOD3 |
| Protein Name / Synonyms | SOD3 (N-term) |
| Clonality | Polyclonal |
| Preparation | This SOD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7-36 amino acids from the N-terminal region of human SOD3. |
| Concentration (lot specific) | 0.300 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC |
| Research Area | Oxidative Stress |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
SOD3 is a member of the superoxide dismutase(SOD) protein family. SODs are antioxidant enzymes that catalyze the dismutation of two superoxide radicals into hydrogen peroxide and oxygen. This protein is thought to protect the brain, lungs, and other tissues from oxidative stress. The protein is secreted into the extracellular space and forms a glycosylated homotetramer that is anchored to the extracellular matrix (ECM) and cell surfaces through an interaction with heparan sulfate proteoglycan and collagen. A fraction of the protein is cleaved near the C-terminus before secretion to generate circulating tetramers that do not interact with the ECM.
Specificity
Other Names
SOD3; Extracellular superoxide dismutase [Cu-Zn]
NCBI Accession #
NP_003093.2
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
25851
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50; FC: 1:10-50
 | Western blot analysis of SOD3 Antibody (N-term) (Cat# 102-16985) in K562 cell line lysates (35ug/lane). SOD3 (arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human lung carcinoma reacted with SOD3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | Flow cytometric analysis of K562 cells using SOD3 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.