Anti-SOX2 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P48431
Gene Id	6657
Gene Symbols	SOX2
Protein Name / Synonyms	SOX2 (N-term)
Clonality	Polyclonal
Preparation	This SOX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-119 amino acids from the N-terminal region of human SOX2.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

The intronless gene for SOX2 encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation.

Specificity

Predicted Reactivity

Mouse, Zebrafish, Chicken, Xenopus

Other Names

SOX2; Transcription factor SOX-2

NCBI Accession #

NP_003097.1

Other Accession #

Q6EJB7; O42569; P48432; Q6P0E1; P48430; Q2PG84; P53783; O00570; O57401; Q2Z1R2; Q6DGL6

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

34310

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; IF: 1:10-50; FC: 1:10-50

	Western blot analysis of SOX2 Antibody (N-term) in 293 cell line lysates (35ug/lane). SOX2(arrow) was detected using the purified Pab.
	Western blot analysis of SOX2 (arrow) using rabbit polyclonal SOX2 Antibody (N-term) (Cat# 102-14836). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SOX2 gene (Lane 2) (Origene Technologies).
	Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SOX2 antibody (N-term) (Cat# 102-14836), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Immunofluorescence analysis of SOX2 Antibody (N-term) (Cat# 102-14836) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
	Flow cytometric analysis of NCI-H292 cells using SOX2 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.