Anti-SOX9 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P48436
Gene Id	6662
Gene Symbols	SOX9
Protein Name / Synonyms	SOX9 (N-term)
Clonality	Polyclonal
Preparation	This SOX9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41-70 amino acids from the N-terminal region of human SOX9.
Concentration (lot specific)	0.280 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF
Research Area	Cardiovascular Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

SOX9 recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal.

Specificity

Predicted Reactivity

Mouse, Pig

Other Names

SOX9; Transcription factor SOX-9

NCBI Accession #

NP_000337.1

Other Accession #

O18896; Q04887

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

56137

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; IF: 1:10-50

	Western blot analysis of anti-SOX9 Antibody (N-term) (Cat# 102-12305) in K562 cell line lysates (35ug/lane). SOX9 (arrow) was detected using the purified Pab.
	Western blot analysis of SOX9 (arrow) using rabbit polyclonal SOX9 Antibody (N-term) (Cat# 102-12305).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SOX9 gene (Lane 2) (Origene Technologies).
	Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with SOX9 antibody (N-term) (Cat# 102-12305), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Immunofluorescence analysis of anti-SOX9 Antibody (N-term) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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